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KMID : 0613820050150050749
Journal of Life Science
2005 Volume.15 No. 5 p.749 ~ p.754
Identification and Cultural Characterization of Antioxidant Producing Bacteria Isolated from the Jeju Coasts
Kim Man-Chul

Heo Moon-Soo
Abstract
An antioxidant- producing bacterium was isolated from sea water in Jeju island. The isolated strain, SC2-1 was gram-positive, catalase positive, facultatively anaerobic, oxidase negative, motile and small rods. The strain utilized sucrose, dextrose, fructose, mannitol and maltose as a sole carbon and energy source and sodium chloride was required for the bacteria growth. The radical scavenging activity of the culture supernatants was determined by DPPH (1,1-diphenyl-2-picrylhydrazyl) method. This bacterium was identified based on cellular fatty acids analysis and 16S rDNA sequencing, and then named Exiguobacterium sp. SC2-1. The modified optimal medium compositions required the addition of maltose 2.5% (w/v), yeast extract 1.5% (w/v) and KH2PO4 0.05% (w/v) in marine broth (Difco. Co. USA). Antioxidant activity of under optimal culture conditions were 93%.
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